| 1. | As a excellent material for preparation of biomembrane , cellulose acetate ( ca ) is a fine carrier for the immobilization of cholesterol oxidase ( cod )
醋酸纤维素( ca )作为一种优异的生物传感用薄膜制备材料，是胆固醇氧化酶( cod )优良的固体载体。 |
| 2. | Therefore , research on cholesterol oxidase has become more and more important . a 1 . 6 kb dna fragment was amplified using pcr method from genomic dna of rhodococcus equi . 4 - 2g2
本研究采用pcr方法从分泌胆固醇氧化酶的马红球菌菌株4 ? 2g2基因组dna中，扩增出特异dna片段，该扩增片段全长约1 . 6kb 。 |
| 3. | Because of its high toxity activity and complementary inseticidal spectrum with bt . endotoxin which is now being used universally , the cholesterol oxidase is acknowleged as one of the representitive of the insecticidal protein of the second generation
因为它的毒杀能力强，而且其杀虫谱又与现在广泛使用的bt .毒蛋白具有一定的互补性，所以被认为是继bt .毒蛋白之后的第二代杀虫蛋白的代表之一。 |
| 4. | As heterologous probe and subsequently show to code for desired enzymatic activity . after a serial of subcloning coupled with southern hybridization and enzymatic activity assay , the functional s . griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2 . 3kb ecori - sall fragment
对phz1140和phz1141进行bamh及bgl的酶谱分析及与choa探针的杂交，将胆同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。 |
| 5. | Moreover , together with cholesterol esterase and catalyse , cholesterol oxidase can be used as test reagent to determine the total cholesterol content in blood plasma for the diagnosis of such as cardiovascular reflex and atherosclerosis clinically
另外，在临床上胆固醇氧化酶可以与胆固醇脂酶和过氧化氢酶一起作为血浆总胆固醇含量的检测试剂，用以诊断冠心病、动脉粥样硬化等心脑血管疾病。因此，对胆固醇氧化酶的研究越来越受到重视。 |
| 6. | The expression mixture were eletrophoresized by sds - page , revealing a specific 56 kd protein in bl21 expression system , which is of the same size in expectation . a colorimetric assay for measuring cholesterol oxidase showed the cholesterol oxidase activity of the expression product
其结果经sds ? page分析表明：以pet - his为表达载体，大肠杆菌bl21 ( de3 ) plyss为宿主菌的重组表达系统表达出约56kd的特异蛋白，蛋白大小与预期结果相一致。 |
| 7. | Cholesterol oxidase is a type of protein that was found to have pest - resistant activity only years ago , its toxity to such sensitive pests as cotton boll weevil is euqivalent to previously found bt . endotoxins . the protein can also inhibit the growth and development of cotton boll worm
胆固醇氧化酶对其敏感害虫?鞘翅目的棉铃象甲的毒杀能力与早期发现的bt .毒蛋白相当，对鳞翅目的一些害虫如烟蚜夜蛾等也有较强的抑制其生长发育的作用。 |
| 8. | In this study , a new gene c / wew , encoding cholesterol oxidase , was isolated from rhodococcus equi . 4 - 2g2 found in china , which may be useful in clinical diagnosis healthy food and pest management in agriculture . in addition , the gene has been expressed successfully , the expression product has cholesterol oxidase activity , thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase
本研究从我国自行分离的马红球菌4 ? 2g2菌株中分离到一种编码胆固醇氧化酶的新基因choew ，它将可应用于临床检测、保健食品和农业抗虫等领域；同时利用原核表达载体成功的在大肠杆菌中表达了目的基因，表现出胆固醇氧化酶酶活，为胆固醇氧化酶的基因工程利用开发作了一定的理论实验基础研究。 |
| 9. | The enzyme retained full activity after being treated at room temperature for 1 hour at ph between 4 . 0 and 11 . 5 . the enzyme can be incubated at 50 for 4h with only less 50 percent loss of activity and is stable in the frozen state . when streptomyces griseus atcc14811 was cultured in 10 . 3 % sucrose yeme liquid medium , production of extracellular cholesterol oxidase increased for 5 days before decrease
利用硫酸铵盐析及deae -纤维素离子交换柱层析提取纯化灰色链霉菌atcc14811发酵上清液中的胆固醇氧化酶，理化性质研究表明酶作用晟适ph为8 . 0 ，最适温度为45 ， ph稳定范围在ph4 . 0 - 11 . 5之间，在50条件下保温4h ，仍保留54酶活力。 |
| 10. | The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size . computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70 . 3 % that would encode a protein of 552 amino acids ( aa ) . the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
对phz1754进行外切核酸酶( exonuclease ， exo )顺序缺失，获得单向长度渐减重叠的系列突变体，核苷酸序列测定显示出该ecor - sal片段的精确大小为2299bps ， frameplot程序分析揭示出该区域一个完整的开放阅读框( orf )的存在，其大小为1656bps ， g + c含量为70 . 3 ，编码552个氨基酸，利用blastsearch程序将orf的核苷酸序列及推导的氨基酸序列与因特网上基因及蛋白质数据库进行综合比较，发现无论在核苷酸水平还是在蛋白水平上，该orf均与胆固醇氧化酶表现出同源性，而且与链霉菌胆固醇氧化酶同源性最高，说明该orf编码胆固醇氧化酶基因。 |
